PGT Agar Cups Recipe (Ez as 123)
For more great Instant Pot recipes, please visit InstantPotEasy.com
Today’s class is an introduction to Agar, what the benefits of using Agar are, and a recipe for how I make my agar cups that you …
You might also like
Leave a Reply
Want to join the discussion?Feel free to contribute!
Could i refrigerate any leftovers so i could melt them back for use later?
Thank You
Thanks
Love the content! You've helped me a ton through out my career. I have a question if you've the time (:
Any idea to the shelf life of this solution? I'm about to use the second half of my solution 8 months later.
A quick question . If the cups are closed the how Will mycelium breathe on agar. I do not think you put any holes in there. Thanks for all that you do. Great show.
What do you do to avoid condensation on the lids from forming/dropping onto the agar?
Good morning! Do you pour about an ounce of agar into each cup?
Could I do this similarly without a PC? I have a large stock pot I use for grain jars. I don't have access to a PC at the moment but I do have every ingredient in the video besides the PC. I'd imagine if I attempted it I would lose pressure and mess it all up. Any info is greatly appreciated.
Many people recommend turning the petri dishes upside after innoculating them… what is your opinion on this, and do you thing it applies to these disposable cups as well?
I accidentally left my spore print unsealed and I was wondering if I can still use it if I’ll spray it with isopropyl alcohol
Any opinion appreciated
Thanks
Can you use LME instead of corn syrup?
I saw this video when you first Posted it yearsss ago and still to the day use Ketchup Cups
Absolutely Smart and effective.
1 cup per 5lb bag of grain and I colonize in 2 weeks
How exactly do you go about keeping the rest of your cups sterile until you use them again?
So not PC not sterile agar?
Can I use malt extract instead of potato flaques ?
Wish I'd have heard of oven tek before! I've had agar and petri dishes for like 4 years but didn't even bother to try this since I don't have a flow hood.
Thanks so much! Now I'll get to know if the old petri dish cultures that have been sitting in my fridge are still alive!
Sterilizing my second batch for transfers today! PGT is the best.
Is it necessary to pressure cook? Is there any other alternative way to sterilize?
Why do we need potato flakes
Can we use something like boiled potatoes or what do you suggest?
I'm not a fan having to buy said when one doesn't have to
What else can we use other than agar
i was going to use small mouth canning jars to use agar, turned on theis side. I have canning jars of all kinds that i use to can my garden. There is also 1 cup wide mouth jars that might also work.
how much ml per agar?
Can I replace the potato flakes with equal amount of LME? Or do the rations change ?
Can you use a temporal thermometer to check the temp?
1st time I've seen the oven tek this way. Very helpful. Wondering how successful the plastic cups are really though.
So, lets say you dont have that 'gun' thermometer, how do you know when it is the right time to pour?
I love this. I haven't been able to find a reasonably priced product to replace the parafilm that everyone uses on their agar plates, and if I can' do it like this and get them a little seal, I won't need to worry about getting parafilm. Thank you so much brother.
🙏🏾
Any reason why I couldn't use small mason jars pre pressure cook? Parcel out the agar mix post boil and pressure cook them separately?
Followed this process today. I found that a small canning jar with the rubber part of the lid facing up to allow pressure equalization worked well in place of a media bottle
Made this recipe to a T, inoculated with spore syringes, a week later and very little growth, some have none.
Any growth that is there is extremely tomentose and growing vertically, the most extreme case is almost 6mm in the air!
Could this be from a lack of nutrients in the agar?
Maybe even too many nutrients and it doesn't need to spread to get anymore.
PGT, help would be appreciated!
Have you ever added antibiotics? Is it really beneficial for agar cups?